The Rural Development Administration (RDA) of Korea has announced the successful development of a genetic identification technology for the Tamnaneubong citrus variety. This technology uses SNP-based KASP markers to distinguish Tamnaneubong from its parent variety Bujihwa and other related citrus types at the DNA level. The patent application for this method, titled ‘SNP-based KASP marker for distinguishing Bujihwa and Tamnaneubong,’ has been completed in Korea. The initiative aims to provide an objective and scientific tool to prevent variety mixing, which is a challenge due to the visual and genetic similarities between Tamnaneubong and Bujihwa.
This advancement directly impacts citrus growers, distributors, and regulatory agencies involved in the domestic and international trade of Korean citrus. Tamnaneubong, registered for plant variety protection in Korea in 2014 and patented in the US in 2019, is one of 23 locally developed citrus varieties. The technology allows for 100% accurate identification using leaf or fruit samples, ensuring the integrity of Tamnaneubong in the supply chain. Other Bujihwa-derived varieties such as M16A, Bipung, Sunnyt, and Saga Kashi 34 are also covered by this method.
The RDA has decoded the entire genomes of Tamnaneubong and Bujihwa using Whole Genome Sequencing (WGS), identifying specific SNPs on chromosomes 2 and 7. These genetic markers are now used in standard PCR-based tests, with plans to transfer marker information and procedures to relevant organizations. In 2025, Tamnaneubong is cultivated on 19.1 hectares in Korea, and the RDA is working to implement these procedures in both domestic and international distribution channels to protect Korean citrus varieties.
Frequently asked questions include: How does the identification process work? Leaf samples are crushed, DNA is extracted, and PCR amplification with specific primers is performed to analyze genetic markers. What is the significance of this technology? It provides an objective method to prevent variety mixing and protect Korean citrus varieties in rapidly changing market environments. The RDA plans to continue research to safeguard the value and stable distribution of Korean citrus varieties amid evolving trade conditions.
Metaqsol opinion: The introduction of SNP-based KASP marker technology by Korea’s RDA is a major advancement in agricultural biotechnology. It provides a reliable, scientific method to distinguish Tamnaneubong from similar citrus varieties, addressing the issue of variety mixing that can undermine market value and intellectual property rights. The policy’s focus on standardization and technology transfer ensures broad adoption and practical impact. This initiative will help maintain the reputation and competitiveness of Korean citrus varieties in both domestic and international markets.